3H4F

Met62Leu variant of nitrite reductase from Alcaligenes faeclis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.2
Spacegroup name	P 21 21 21
Unit cell lengths	61.132, 102.223, 146.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.520 - 2.100
R-factor	0.1695
Rwork	0.166
R-free	0.22698
Structure solution method	Refinement of wild-type structure
Starting model (for MR)	1sjm
RMSD bond length	0.010
RMSD bond angle	1.282
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.520	84.520
High resolution limit [Å]	2.100	2.100
Number of reflections	50095

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	298	pH 4.5, VAPOR DIFFUSION, temperature 298K