3H4C

Structure of the C-terminal Domain of Transcription Factor IIB from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2008-06-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	109.233, 109.233, 51.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 2.300
R-factor	0.20831
Rwork	0.204
R-free	0.24938
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.065
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0036)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	13573
Completeness [%]	94.8	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	24% PEG 3350, 0.2M sodium chloride, 0.1M Bis-tris (2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol), pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	24% PEG 3350, 0.2M sodium chloride, 0.1M Bis-tris (2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol), pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	24% PEG 3350, 0.2M sodium chloride, 0.1M Bis-tris (2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol), pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K