3H47
X-ray Structure of Hexameric HIV-1 CA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Collection date | 2008-07-18 |
Wavelength(s) | 1.000 |
Spacegroup name | P 6 |
Unit cell lengths | 90.995, 90.995, 56.853 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.740 - 1.900 |
R-factor | 0.2342 |
Rwork | 0.232 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 2PXR - with residues 1-14 and 87-100 deleted PDB 2BUO - with residues 175-190 deleted |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 21352 |
<I/σ(I)> | 21.1 |
Completeness [%] | 99.9 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 10-12% PEG 8,000, 100 mM sodium malonate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |