3H43
N-terminal domain of the proteasome-activating nucleotidase of Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791, 0.9792, 0.9641 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 126.640, 129.620, 60.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.890 - 2.100 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 58671 | |
| Completeness [%] | 98.8 | 91.3 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 0.1 M NaOAc, pH 4.5, 0.2 M Li2SO4and 34%-36% MPD (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
