3H3V
Yeast RNAP II containing poly(A)-signal sequence in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918905 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.470, 391.620, 284.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y1w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.250 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 202368 | |
| <I/σ(I)> | 9.6 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 295 | 200 mM ammonium acetate, 50mM HEPES pH 7.0, 4% polyethylene glycol 6000, 5mM TCEP (tris (2-carboxyethyl) phosphine hydrochloride), VAPOR DIFFUSION, HANGING DROP, temperature 295.0 K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 2 | ammonium acetate | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 2 | HEPES | ||
| 5 | 1 | 1 | polyethylene glycol 6000 | ||
| 6 | 1 | 2 | polyethylene glycol 6000 | ||
| 7 | 1 | 1 | TCEP (tris (2-carboxyethyl) phosphine hydrochloride) | ||
| 8 | 1 | 2 | TCEP (tris (2-carboxyethyl) phosphine hydrochloride) |
