3H3P
Crystal structure of HIV epitope-scaffold 4E10 Fv complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 107 |
Detector technology | IMAGE PLATE |
Collection date | 2008-08-13 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.400, 92.400, 272.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.080 - 2.700 |
R-factor | 0.23171 |
Rwork | 0.229 |
R-free | 0.28697 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | computationally-derived model of the Fv based on 1TZG |
RMSD bond length | 0.008 |
RMSD bond angle | 1.109 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.020 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.104 | 0.347 |
Number of reflections | 19557 | |
<I/σ(I)> | 7.5 | 2.9 |
Completeness [%] | 98.6 | 99.6 |
Redundancy | 4.56 | 4.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.2 M calcium acetate, 8% PEG 8000, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |