3H3N
Glycerol Kinase H232R with Glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.812, 200.464, 56.505 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.730 |
R-factor | 0.216 |
Rwork | 0.207 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xup |
RMSD bond length | 0.005 |
RMSD bond angle | 1.272 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.730 | 1.700 |
Number of reflections | 115245 | |
<I/σ(I)> | 9.3 | 2.8 |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | KH2PO4, PEG 8000, vapor diffusion, temperature 298K |