3H3M
Crystal structure of flagellar protein FliT from Bordetella bronchiseptica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 67.751, 67.751, 82.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.400 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.651 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.109 | 0.512 |
| Number of reflections | 6894 | |
| <I/σ(I)> | 18.939 | 2.9 |
| Completeness [%] | 97.3 | 98.2 |
| Redundancy | 9.4 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M POTASSIUM THIOCYANATE,30% PEG 2K MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
