3H3H

Crystal structure of a snoal-like protein of unknown function (bth_ii0226) from burkholderia thailandensis e264 at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97889,0.97833
Spacegroup name	C 2 2 21
Unit cell lengths	61.746, 73.399, 97.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.424 - 1.600
R-factor	0.18
Rwork	0.179
R-free	0.20200
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.424	29.424	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.041	0.025	0.563
Number of reflections	29144	5352	5056
<I/σ(I)>	10.17	25.5	1.4
Completeness [%]	96.1	93.2	85.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	27.6000% polyethylene glycol 6000, 0.1M MES pH 5.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K