3H3D
Drosophila Pumilio RNA binding domain (Puf domain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 63 |
| Unit cell lengths | 94.580, 94.580, 229.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.780 - 2.300 |
| R-factor | 0.254 |
| Rwork | 0.252 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.610 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.089 | 0.311 |
| Number of reflections | 49178 | |
| <I/σ(I)> | 20.7 | 2.4 |
| Completeness [%] | 95.8 | 73.2 |
| Redundancy | 8.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 2.1M Ammonium sulfate, 100mM HEPES, 4% DMSO, 2mM DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 2 |
