3H36
Structure of an uncharacterized domain in polyribonucleotide nucleotidyltransferase from Streptococcus mutans UA159
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97945,0.97931 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.312, 59.312, 93.090 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.990 - 1.800 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.538 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.082 | 0.671 |
Number of reflections | 11267 | |
<I/σ(I)> | 65.5 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 22.7 | 22.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1M Na Cacodylate pH6.5,0.2M Ca Acetate, 40% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |