3H31
Structure of Rhodothermus marinus HiPIP at 1.0 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.267, 43.343, 46.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.000 |
R-factor | 0.1044 |
Rwork | 0.104 |
R-free | 0.14400 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 0.042 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.800 | 1.050 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.128 | 0.396 |
Number of reflections | 30715 | |
<I/σ(I)> | 9.2 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | ammonium sulfate, dioxane, sodium citrate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | ammonium sulfate, dioxane, sodium citrate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |