3H31
Structure of Rhodothermus marinus HiPIP at 1.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.267, 43.343, 46.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| R-factor | 0.1044 |
| Rwork | 0.104 |
| R-free | 0.14400 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.042 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.128 | 0.396 |
| Number of reflections | 30715 | |
| <I/σ(I)> | 9.2 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | ammonium sulfate, dioxane, sodium citrate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | ammonium sulfate, dioxane, sodium citrate, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
