3H2T
Crystal structure of gene product 6, baseplate protein of bacteriophage T4
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97948 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.774, 94.579, 136.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.856 - 3.200 |
R-factor | 0.2344 |
Rwork | 0.230 |
R-free | 0.32160 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.215 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.096 | 0.476 |
Number of reflections | 16878 | |
<I/σ(I)> | 26.9 | 3.9 |
Completeness [%] | 99.6 | 97 |
Redundancy | 7.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 1.2 M Ammonium Sulfate, buffered with HEPES, pH 7.0, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 7 | 298 | 1.2 M Ammonium Sulfate, buffered with HEPES, pH 7.0, VAPOR DIFFUSION, temperature 298K |