3H2J

Crystal structure of the rice cell wall degrading esterase LipA from Xanthomonas oryzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Detector technology	IMAGE PLATE
Collection date	2006-12-18
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	93.130, 62.370, 66.150
Unit cell angles	90.00, 90.87, 90.00

Refinement procedure

Resolution	24.200 - 1.890
R-factor	0.193
Rwork	0.193
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h2g
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.890
Number of reflections	27294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	48% PEG 400, 0.10M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K