3H2G
Crystal structure of a rice cell wall degrading esterase LipA from Xanthomonas oryzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.660, 54.680, 66.330 |
Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
Resolution | 24.500 - 1.860 |
R-factor | 0.161 |
Rwork | 0.161 |
R-free | 0.18700 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 30946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 12% PEG 6000, 0.10M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |