3H2B

Crystal structure of the SAM-dependent methyltransferase cg3271 from Corynebacterium glutamicum in complex with S-adenosyl-L-homocysteine and pyrophosphate. Northeast Structural Genomics Consortium Target CgR113A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.97869
Spacegroup name	P 21 21 21
Unit cell lengths	70.827, 72.424, 74.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 2.000
R-factor	0.174
Rwork	0.173
R-free	0.22600
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.121	0.525
Number of reflections	50463
<I/σ(I)>	14.14	2.62
Completeness [%]	100.0	100
Redundancy	5.4	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH UNDER OIL	6.5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5. Reservoir solution: 100mM Sodium cacodylate pH 6.5, 25% PEG 8000, 200mM Ammonium sulfate, MICROBATCH UNDER OIL, temperature 277K