3H1Z
Molecular basis for the association of PIPKIgamma -p90 with the clathrin adaptor AP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.561, 83.469, 91.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.250 - 1.830 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g30 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.618 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 3.940 | 1.830 |
| Rmerge | 0.060 | 0.028 | 0.461 |
| Number of reflections | 24788 | ||
| <I/σ(I)> | 20.383 | ||
| Completeness [%] | 94.6 | 97.1 | 88.1 |
| Redundancy | 4.5 | 4.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 18% PEG8000, 100mM HEPES, pH7.5, 4mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
