3H1M
Crystal structure of mannose 6-phosphate isomerase (holo; zinc bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.030, 92.460, 111.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.990 - 2.500 |
| R-factor | 0.18999 |
| Rwork | 0.188 |
| R-free | 0.23213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.423 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.433 |
| Number of reflections | 12581 | |
| <I/σ(I)> | 7.6 | 2.6 |
| Completeness [%] | 93.6 | 90 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Micro-bactch under oil | 6.5 | 293 | 0.1 M magnesium acetate, 0.2M sodium cacodylate pH 6.5, 20% PEG 8000, 5% dioxane, Micro-bactch under oil, temperature 293K |
