3H1F
Crystal structure of CheY mutant D53A of Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.335, 38.081, 38.639 |
| Unit cell angles | 90.00, 107.35, 90.00 |
Refinement procedure
| Resolution | 15.530 - 2.200 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.23256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwg |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.828 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.880 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.240 |
| Number of reflections | 4993 | |
| <I/σ(I)> | 10.6 | 4.5 |
| Completeness [%] | 98.5 | 97 |
| Redundancy | 3.78 | 3.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1M sodium acetate, 35% MPEG2000, 0.05M ammonium sulfate, 1mM Magnesium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
