3H1E
Crystal structure of Mg(2+) and BeH(3)(-)-bound CheY of Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.956, 38.098, 38.885 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 33.320 - 2.400 |
| R-factor | 0.15449 |
| Rwork | 0.151 |
| R-free | 0.22013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwg |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.395 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.320 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.019 | 0.029 |
| Number of reflections | 4888 | |
| <I/σ(I)> | 55.1 | 39 |
| Completeness [%] | 95.3 | 91.8 |
| Redundancy | 4.27 | 4.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1M Sodium acetate, 35% MPEG2000, 0.05M Ammonium sulfate, 1mM Magnesium chloride, 1:10 beryllium, 1:100 sodium fluoride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
