3H14
Crystal structure of a putative aminotransferase from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.367, 86.376, 144.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.810 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.810 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 29683 | |
| <I/σ(I)> | 17 | 6.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.6 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 200mM Calcium acetate, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
