3H0D
Crystal structure of CtsR in complex with a 26bp DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.198, 76.087, 167.568 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.213 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SOLOMON |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.725 | 25.730 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.021 | 0.475 | |
| Total number of observations | 3320 | 15912 | |
| Number of reflections | 32628 | ||
| <I/σ(I)> | 5.424 | 23.3 | 1.5 |
| Completeness [%] | 99.6 | 96.5 | 99.9 |
| Redundancy | 3.3 | 3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 220mM ammonium sulfate, 20% PEG300, 15% glycerol, 100mM phosphate/citrate, pH 4.2, vapor diffusion, sitting drop, temperature 298K |
