3H00
Structure of the C-terminal Domain of a Putative HIV-1 gp41 Fusion Intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.764, 47.499, 74.824 |
Unit cell angles | 90.00, 128.32, 90.00 |
Refinement procedure
Resolution | 40.100 - 2.200 |
R-factor | 0.21403 |
Rwork | 0.212 |
R-free | 0.25526 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gwo |
RMSD bond length | 0.029 |
RMSD bond angle | 2.406 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.100 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.044 | 0.269 |
Number of reflections | 16151 | |
<I/σ(I)> | 18.7 | 3.1 |
Completeness [%] | 93.7 | 76.2 |
Redundancy | 3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 295 | 3% PEG8000, 0.1M Tris-HCl, 0.1M cesium chloride, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |