3GZE
Algal prolyl 4-hydroxylase complexed with zinc and (Ser-Pro)5 peptide substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.932 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.250, 58.750, 105.160 |
Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
Resolution | 20.600 - 1.980 |
R-factor | 0.21888 |
Rwork | 0.217 |
R-free | 0.25392 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v4a |
RMSD bond length | 0.020 |
RMSD bond angle | 1.813 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.040 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.062 | 0.201 |
Number of reflections | 68688 | |
<I/σ(I)> | 12.16 | 4.3 |
Completeness [%] | 93.4 | 87.1 |
Redundancy | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under-oil | 5.5 | 295 | 5% PEG Smear, 0.1M acetate, 10mM zinc acetate, pH 5.5, Microbatch under-oil, temperature 295K |