Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.218, 85.813, 188.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.100 |
| R-factor | 0.18224 |
| Rwork | 0.179 |
| R-free | 0.23499 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p3w |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.698 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.107 | 0.370 |
| Number of reflections | 54053 | |
| <I/σ(I)> | 13.1 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 100mM HEPES, 10% PEG 6000, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
