3GYD
Crystal structure of a cyclic nucleotide-binding domain (mfla_1926) from methylobacillus flagellatus kt at 1.79 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-18 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97956, 0.97944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.032, 39.745, 70.977 |
Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
Resolution | 29.374 - 1.790 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.21200 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.569 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.374 | 29.374 | 1.840 |
High resolution limit [Å] | 1.790 | 8.010 | 1.790 |
Rmerge | 0.090 | 0.041 | 0.559 |
Total number of observations | 1070 | 4786 | |
Number of reflections | 30961 | ||
<I/σ(I)> | 6.144 | 15.2 | 1.1 |
Completeness [%] | 99.1 | 96 | 90.8 |
Redundancy | 3 | 2.9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 277 | NANODROP, 36.0% PEG 3000, 0.1M CHES pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |