3GYB
Crystal structure of a LacI-family transcriptional regulatory protein from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.073, 61.882, 65.604 |
| Unit cell angles | 115.83, 106.37, 90.50 |
Refinement procedure
| Resolution | 32.550 - 1.600 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.550 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.326 |
| Number of reflections | 63137 | |
| <I/σ(I)> | 13.6 | 2.5 |
| Completeness [%] | 93.5 | 65.5 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Magnesium acetate tetrahydrate 0.1M Sodium cacodylate trihydrate 20% Polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
