3GXV

Three-dimensional structure of N-terminal domain of DnaB Helicase from Helicobacter pylori and its interactions with primase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-18
Detector	RAYONIX MX-225
Wavelength(s)	0.997
Spacegroup name	P 21 21 21
Unit cell lengths	71.830, 78.150, 108.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.320 - 2.200
R-factor	0.249
Rwork	0.249
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r5u
RMSD bond length	0.007
RMSD bond angle	1.800
Data reduction software	AUTOMAR
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.320	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.105	0.575
Number of reflections	30563
<I/σ(I)>	9.78	2.35
Redundancy	5.32	3.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	Bis Tris, Ammonium Sulphate, Sodium malonate, Peg 550MME, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K