3GXQ
Structure of ArtA and DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11588 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.830, 61.130, 40.500 |
| Unit cell angles | 90.00, 117.32, 90.00 |
Refinement procedure
| Resolution | 35.980 - 2.350 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.26100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.080 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.074 | 0.258 |
| Number of reflections | 6644 | |
| <I/σ(I)> | 11.5 | 2.3 |
| Completeness [%] | 93.1 | 73.7 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 10% PEG 2000, 0.1M Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 | ||
| 2 | 1 | 1 | Acetate | ||
| 3 | 1 | 2 | PEG 2000 | ||
| 4 | 1 | 2 | Acetate |
