3GXF
Crystal structure of acid-beta-glucosidase with isofagomine at neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.311, 92.013, 152.362 |
| Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
| Resolution | 32.120 - 2.400 |
| R-factor | 0.182 |
| Rwork | 0.178 |
| R-free | 0.24500 |
| Starting model (for MR) | '2NSX |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.957 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.120 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 103572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.8 M NaH2PO4, 0.8 M KH2PO4, 0.1 M Hepes pH 7.5, SOAK with 500uM isofagomine for 10 min, glycerol in cryo, VAPOR DIFFUSION, temperature 298K |
