3GXE
Complex of a Low Affinity Collagen Site with the Fibronectin 8-9FnI Domain Pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.570, 56.570, 152.661 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.648 - 2.600 |
| R-factor | 0.223 |
| Rwork | 0.219 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ejh Chain A and part of chain E |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.683 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | PHENIX (2009_02_15_2320_3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.676 | 2.740 |
| High resolution limit [Å] | 2.558 | 2.600 |
| Rmerge | 0.074 | 0.442 |
| Total number of observations | 8589 | |
| Number of reflections | 16804 | |
| <I/σ(I)> | 14.5 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.1 | 0.1M HEPES pH 7.5, 4.3M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293.1K |
