3GX1
Crystal structure of a domain of lin1832 from Listeria innocua
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 152.852, 152.852, 45.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.740 - 2.300 |
| R-factor | 0.21307 |
| Rwork | 0.212 |
| R-free | 0.24088 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.928 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.577 |
| Number of reflections | 23117 | |
| <I/σ(I)> | 45.5 | 3.8 |
| Completeness [%] | 94.2 | 71.3 |
| Redundancy | 13.4 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 1.6M MgSO4, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
