3GX0
Crystal Structure of GSH-dependent Disulfide bond Oxidoreductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.490, 68.490, 111.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.1935 |
Rwork | 0.191 |
R-free | 0.24981 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.662 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.130 | 0.551 |
Number of reflections | 12571 | |
<I/σ(I)> | 17.5 | 3.35 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 10.9 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | Protein in 20 mM potassium dihydrogen phosphate, 2 mM DTT, 1mM EDTA, 10 mM glutathione. Well solution: 20% w/v PEG 3000, 100 mM acetate., VAPOR DIFFUSION, HANGING DROP |