3GWO
Structure of the C-terminal Domain of a Putative HIV-1 gp41 Fusion Intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2003-07-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793,0.9796,0.9681 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 33.957, 33.957, 174.838 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.700 - 1.650 |
R-factor | 0.23288 |
Rwork | 0.232 |
R-free | 0.25930 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.722 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.700 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.062 | 0.485 |
Number of reflections | 14912 | |
<I/σ(I)> | 15.6 | 5.2 |
Completeness [%] | 99.3 | 100 |
Redundancy | 8.4 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 28% Jeffamine M600, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |