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3GWO

Structure of the C-terminal Domain of a Putative HIV-1 gp41 Fusion Intermediate

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X4A
Synchrotron siteNSLS
BeamlineX4A
Temperature [K]95
Detector technologyCCD
Collection date2003-07-02
DetectorADSC QUANTUM 4
Wavelength(s)0.9793,0.9796,0.9681
Spacegroup nameP 31 2 1
Unit cell lengths33.957, 33.957, 174.838
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution20.700 - 1.650
R-factor0.23288
Rwork0.232
R-free0.25930
Structure solution methodMAD
RMSD bond length0.018
RMSD bond angle1.722
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.7001.710
High resolution limit [Å]1.6501.650
Rmerge0.0620.485
Number of reflections14912
<I/σ(I)>15.65.2
Completeness [%]99.3100
Redundancy8.48.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP629528% Jeffamine M600, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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