3GWL

Crystal structure of ASFV pB119L, a viral sulfhydryl oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-09-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.450, 69.000, 83.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.218
R-free	0.26100
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	2.130
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	16153
<I/σ(I)>	13.9	5.1
Completeness [%]	98.8	100
Redundancy	7.3	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1M HEPES (pH 7.8), 7% (v/v) isopropanol, 5% (w/v) PEG 12K, VAPOR DIFFUSION, HANGING DROP, temperature 293K