3GWK
Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.900 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 76.580, 76.580, 149.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.096 - 1.300 |
R-factor | 0.148 |
Rwork | 0.147 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gvm |
RMSD bond length | 0.017 |
RMSD bond angle | 1.318 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.053 | 0.470 |
Number of reflections | 62862 | 4438 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 97.6 | 95.2 |
Redundancy | 10.8 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 0.1M Tris-HCl pH 8.0, 1.9M (NH4)SO4, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K |