3GWI
Crystal Structure of Mg-ATPase Nucleotide binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-28 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0379 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 99.620, 99.620, 46.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.19214 |
Rwork | 0.191 |
R-free | 0.21981 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.158 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.630 | 1.610 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.042 | 0.206 |
Number of reflections | 33678 | |
<I/σ(I)> | 28.1 | 10.1 |
Completeness [%] | 94.7 | 70 |
Redundancy | 7.5 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 2% PEG 400, 2M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |