3GVX
Crystal structure of Glycerate dehydrogenase related protein from Thermoplasma acidophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-23 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.512, 77.842, 152.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.420 - 2.200 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.26100 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.200 | 2.210 |
Rmerge | 0.094 | 0.490 |
Number of reflections | 38525 | |
<I/σ(I)> | 14.8 | 11 |
Completeness [%] | 94.4 | 71.8 |
Redundancy | 8.2 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 298 | 0.2M Pottasium phosphate dibasic, 25% peg 3350, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |