3GVT
Structure and RNA binding of the mouse Pumilio-2 Puf Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 63 |
| Unit cell lengths | 150.540, 150.540, 77.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.870 - 2.800 |
| R-factor | 0.19457 |
| Rwork | 0.192 |
| R-free | 0.25317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gvo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.328 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.880 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.471 |
| Number of reflections | 24746 | |
| <I/σ(I)> | 11.5 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2M ammonium citrate, 0.2M Li2SO4, 22%(w/v) PEG 4000 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
