3GVN
The 1.2 Angstroem crystal structure of an E.coli tRNASer acceptor stem microhelix reveals two magnesium binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 35.793, 39.126, 31.367 |
| Unit cell angles | 90.00, 111.08, 90.00 |
Refinement procedure
| Resolution | 120.000 - 1.200 |
| Rwork | 0.190 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v6w |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.074 | 0.154 |
| Number of reflections | 12806 | |
| <I/σ(I)> | 18.7 | 1.8 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 7.1 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 10% (v/v) MPD, 40mM sodium cacodylate, 20mM cobalt hexamine, 80mM sodium chloride, 20mM magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
