3GVL
Crystal Structure of endo-neuraminidaseNF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 |
Unit cell lengths | 119.092, 119.092, 175.981 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.640 - 1.410 |
R-factor | 0.16946 |
Rwork | 0.169 |
R-free | 0.18769 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.434 |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.5.0039) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.640 | 1.450 | |
High resolution limit [Å] | 1.410 | 6.310 | 1.410 |
Rmerge | 0.074 | 0.025 | 0.530 |
Number of reflections | 184838 | ||
<I/σ(I)> | 38.1 | 2.4 | |
Completeness [%] | 99.7 | 95.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 20% (w/v) PEG 8000, 0.1M Tris/HCl, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |