3GVH
Crystal structure of Lactate/malate dehydrogenase from Brucella melitensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-25 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.750, 114.350, 148.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.000 - 2.300 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.21800 |
Structure solution method | MR |
Starting model (for MR) | 1sow modified with ccp4 program chainsaw |
RMSD bond length | 0.018 |
RMSD bond angle | 1.585 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.620 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.097 | 0.610 |
Number of reflections | 69983 | |
<I/σ(I)> | 16.9 | 2.1 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | JCSG+ SCREEN, D12: 40MM K-PO4, 16% PEG 8000, 20% GLYCEROL, BRABA.00005.A AT 28.5MG/ML, VAPOR DIFFUSION, SITTING DROP, temperature 290K |