3GV0
Crystal structure of LacI family transcription regulator from Agrobacterium tumefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 86.710, 86.710, 111.224 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.360 - 2.350 |
R-factor | 0.20113 |
Rwork | 0.198 |
R-free | 0.25450 |
Structure solution method | SAD |
RMSD bond length | 0.025 |
RMSD bond angle | 1.992 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.360 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.124 | 0.423 |
Number of reflections | 20658 | |
<I/σ(I)> | 15.8 | 4.8 |
Completeness [%] | 99.8 | 97.6 |
Redundancy | 18.9 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 20% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |