3GUW
Crystal Structure of the TatD-like Protein (AF1765) from Archaeoglobus fulgidus, Northeast Structural Genomics Consortium Target GR121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.069, 72.192, 89.163 |
| Unit cell angles | 89.87, 89.74, 82.73 |
Refinement procedure
| Resolution | 19.810 - 3.200 |
| R-factor | 0.243 |
| Rwork | 0.241 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD (& SOLVE/RESOLVE) |
| Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.087 | 0.245 |
| Number of reflections | 32519 | |
| <I/σ(I)> | 9.3 | 2.19 |
| Completeness [%] | 97.5 | 98.1 |
| Redundancy | 1.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M sodium acetate, 1.5M (NH4)2SO4, and 15% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 291K |
