3GUV
Crystal structure of a resolvase family site-specific recombinase from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.991, 46.991, 119.259 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.29100 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.519 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.225 |
| Total number of observations | 7007 | |
| Number of reflections | 8232 | |
| <I/σ(I)> | 14.1 | 5.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 20% PEG 3350, 200mM zinc acetate, pH 7.0, vapor diffusion, temperature 294K |
