3GUH
Crystal Structure of Wild-type E.coli GS in complex with ADP and DGM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2005-05-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 125.841, 125.841, 153.263 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.790 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzu |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.276 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.130 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.146 | 0.516 |
| Number of reflections | 29543 | |
| <I/σ(I)> | 23.996 | |
| Completeness [%] | 99.2 | 92.9 |
| Redundancy | 8.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 277 | 40% (w/v) PEG 4000, 0.2 M NaAc, 0.1 M HEPPSO, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
