3GU0
Promiscuous Substrate Recognition in Folding and Assembly Activities of the Trigger Factor Chaperone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97903 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.387, 114.487, 94.558 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.500 |
R-factor | 0.25 |
Rwork | 0.244 |
R-free | 0.35800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.226 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 3.570 |
High resolution limit [Å] | 3.450 | 7.320 | 3.450 |
Rmerge | 0.081 | 0.041 | 0.321 |
Number of reflections | 6747 | ||
<I/σ(I)> | 17.492 | ||
Completeness [%] | 93.7 | 88.9 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10-15% PEG4000, 200 MgCl2, 100 Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |