3GTY
Promiscuous Substrate Recognition in Folding and Assembly Activities of the Trigger Factor Chaperone
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9792,0.9794,0.9687 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 94.162, 94.162, 193.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 3.400 |
R-factor | 0.261 |
Rwork | 0.257 |
R-free | 0.32900 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.321 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
Rmerge | 0.057 | 0.022 | 0.369 |
Number of reflections | 13240 | ||
<I/σ(I)> | 21.369 | ||
Completeness [%] | 98.4 | 88.9 | 99.9 |
Redundancy | 3.3 | 3.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 5-10 % PEG4000, 100 NaOAc, pH 5.5, vapor diffusion, hanging drop, temperature 298K |