3GTU
LIGAND-FREE HETERODIMERIC HUMAN GLUTATHIONE S-TRANSFERASE M2-3 (EC 2.5.1.18), MONOCLINIC CRYSTAL FORM
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 289 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-07 |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.970, 102.747, 103.977 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gtu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 21.110 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.113 | 0.341 |
| Number of reflections | 25387 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 94.4 | 69.4 |
| Redundancy | 2.4 | 1.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | pH 7.5 |
