3GTP
Backtracked RNA polymerase II complex with 24mer RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.778, 222.113, 194.854 |
| Unit cell angles | 90.00, 102.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.900 |
| R-factor | 0.2676 |
| Rwork | 0.266 |
| R-free | 0.29885 |
| Structure solution method | MOLECULA REPLACEMENT |
| Starting model (for MR) | 3gtg |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.808 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 56095 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 93.3 | 82.5 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM Dioxane, 10mM DTT, 9-11% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2HPO4/NaH2PO4 | ||
| 2 | 1 | 1 | Dioxane | ||
| 3 | 1 | 1 | DTT | ||
| 4 | 1 | 1 | PEG 6000 | ||
| 5 | 1 | 2 | (NH4)2HPO4/NaH2PO4 | ||
| 6 | 1 | 2 | Dioxane | ||
| 7 | 1 | 2 | DTT | ||
| 8 | 1 | 2 | PEG 6000 |
